{"created":"2023-05-15T08:55:46.479354+00:00","id":996,"links":{},"metadata":{"_buckets":{"deposit":"a6a600de-e15f-4ac7-814d-3f1466c97d14"},"_deposit":{"created_by":10,"id":"996","owners":[10],"pid":{"revision_id":0,"type":"depid","value":"996"},"status":"published"},"_oai":{"id":"oai:tsurumi-u.repo.nii.ac.jp:00000996","sets":["100:101:103:149"]},"author_link":["754"],"item_10007_date_8":{"attribute_name":"報告年度","attribute_value_mlt":[{"subitem_date_issued_datetime":"2021-03-31","subitem_date_issued_type":"Issued"}]},"item_10007_description_13":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"研究成果の概要（和文）： Ca結合性アネキシンの1つAnxa5は腱・靭帯の骨付着部(enthesis)に局在し、KOマウスではenthesisで骨増大が起きる。本研究では①Anxa5をin vitroでノックダウン後、遺伝子発現の解析、②KOマウスで石灰化制御因子の解析から、enthesisでのAnxa5の石灰化制御にはピロリン酸制御因子と機械的刺激が介在する可能性を見出した。enthesisで骨増大を示すKOマウスはうつ病様行動を示すが、新たに運動制御機能の低下を見出した。この表現型は中枢細胞でのAnxa5欠損に起因することが示唆された。KOマウスでの表現型に対しリコンビナントタンパクによるレスキューが課題である。","subitem_description_language":"ja","subitem_description_type":"Abstract"},{"subitem_description":"研究成果の概要（英文）：One of the Ca binding proteins, Annexin group, Anxa5 is localized to bone attachments of tendon and ligament (enthesis). We have found that Anxa5 KO mice have bone overgrowths at entheses. The present analyses of gene expression in knockdown cells and immuno-histology using tissue sections of KO mice showed that mineralization at the enthesis may be regulated by pyrophosphates and/or mechanical loading. Anxa5 KO mice with bone overgrowths at entheses exhibit depression behavior. A behavior testing revealed that KO mice exhibit decreases of regulation of physical function. We suggest that this phenotype is due to deletions of Anxa5 localizations in the central nervous system. Rescue experiment with recombinant protein for the phenotypes of KO mice would be required.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10007_description_14":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"・2020(令和2)年度 科学研究費補助金 基盤研究(C) 研究成果報告書","subitem_description_language":"ja","subitem_description_type":"Other"},{"subitem_description":"・研究期間 (年度)：2018-04-01 – 2021-03-31","subitem_description_language":"ja","subitem_description_type":"Other"},{"subitem_description":"・研究分野：硬組織石灰化制御の薬理学的応用","subitem_description_language":"ja","subitem_description_type":"Other"},{"subitem_description":"・研究成果の学術的意義や社会的意義 : 学術的意義：①腱・靭帯の骨への付着部は運動機能の上で重要な機能を担っている。本研究によりAnxa5の末梢、中枢における機能を介したenthesisへの関与を明らかにすることは、enthesisの中枢・末梢における制御機構の解明に寄与できる。②マウスにおけるAnxa5のうつ病様行動への影響のメカニズムを明らかにすることにより、海外研究協力者が同定したヒトにおける遺伝子変異を持った患者の病態発症メカニズム解明に寄与できる。社会的意義：Anxa5リコンビナントタンパク投与によるレスキューから得られる結果は、ヒトAnxa5欠損による病態の治療法に応用できる可能性を有すると言う点で、社会的意義が高い。","subitem_description_language":"ja","subitem_description_type":"Other"}]},"item_10007_description_9":{"attribute_name":"研究課題番号","attribute_value_mlt":[{"subitem_description":"研究課題/領域番号 : 18K09757","subitem_description_language":"ja","subitem_description_type":"Other"}]},"item_10007_relation_17":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-18K09757/","subitem_relation_type_select":"URI"}}]},"item_10007_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-03-02"}],"displaytype":"detail","filename":"18K09757.pdf","filesize":[{"value":"82.4 kB"}],"format":"application/pdf","licensetype":"license_6","mimetype":"application/pdf","url":{"label":"18K09757","url":"https://tsurumi-u.repo.nii.ac.jp/record/996/files/18K09757.pdf"},"version_id":"8ed1a9cd-cff8-4fd2-b3cc-3499f7417be9"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"腱・靱帯の骨接合部","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"アネキシン","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"荷重負荷","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"石灰化制御因子","subitem_subject_language":"ja","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_researcher":{"attribute_name":"研究代表者","attribute_type":"creator","attribute_value_mlt":[{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"","affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"http://www.isni.org/isni/"}],"affiliationNames":[{"affiliationName":"","affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"KOMATSU, Koichiro","creatorNameLang":"en"},{"creatorName":"小松, 浩一郎","creatorNameLang":"ja"},{"creatorName":"コマツ, コウイチロウ","creatorNameLang":"ja-Kana"}],"familyNames":[{"familyName":"KOMATSU","familyNameLang":"en"},{"familyName":"小松","familyNameLang":"ja"},{"familyName":"コマツ","familyNameLang":"ja-Kana"}],"givenNames":[{"givenName":"Koichiro","givenNameLang":"en"},{"givenName":"浩一郎","givenNameLang":"ja"},{"givenName":"コウイチロウ","givenNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"754","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"60153665","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=60153665"},{"nameIdentifier":"754","nameIdentifierScheme":"WEKO著者ID"}]}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"アネキシンA5の中枢と末梢における機能とAAV投与による遺伝子治療への展開","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"アネキシンA5の中枢と末梢における機能とAAV投与による遺伝子治療への展開","subitem_title_language":"ja"},{"subitem_title":"Functional roles of Anxa5 in the central and peripheral nervous systems and application of AAV administration to gene therapy","subitem_title_language":"en"}]},"item_type_id":"10007","owner":"10","path":["149"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2022-03-02"},"publish_date":"2022-03-02","publish_status":"0","recid":"996","relation_version_is_last":true,"title":["アネキシンA5の中枢と末梢における機能とAAV投与による遺伝子治療への展開"],"weko_creator_id":"10","weko_shared_id":-1},"updated":"2024-08-01T07:59:39.746457+00:00"}