{"created":"2025-05-09T05:30:29.218574+00:00","id":2000189,"links":{},"metadata":{"_buckets":{"deposit":"73792d1a-f80b-4e36-bbcc-69cd32bb8de6"},"_deposit":{"created_by":10,"id":"2000189","owners":[10],"pid":{"revision_id":0,"type":"depid","value":"2000189"},"status":"published"},"_oai":{"id":"oai:tsurumi-u.repo.nii.ac.jp:02000189","sets":["100:101:103:1744946052441"]},"author_link":["772"],"item_10007_date_8":{"attribute_name":"報告年度","attribute_value_mlt":[{"subitem_date_issued_datetime":"2024-03-31","subitem_date_issued_type":"Issued"}]},"item_10007_description_13":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"研究成果の概要（和文）：ジフテリアトキシン受容体（hHB-EGF）を唾液腺だけに発現させた遺伝子改変マウス（MUC7p-TRECK）を作出し、ジフテリアトキシン投与による唾液腺特異的な組織傷害および唾液分泌量低下の程度の評価と、脂肪由来幹細胞移植による唾液腺組織再生の機序を検討した。遺伝子改変マウスへのジフテリアトキシン投与により唾液腺特異的にアポトーシスが生じ、唾液分泌量の低下が確認された。また、遺伝子改変マウスと同系統であるC57BL/6マウスから間質血管細胞群（脂肪由来幹細胞を豊富に含む細胞群）を単離した。その過程で間質血管細胞群は継代培養を進めるに伴い高度の細胞老化が生じる可能性が示唆された。","subitem_description_language":"ja","subitem_description_type":"Abstract"},{"subitem_description":"研究成果の概要（英文）：To investigate the mechanism of salivary gland tissue regeneration by adipose-derived stem cell transplantation, genetically modified mice (MUC7p-TRECK) expressing the diphtheria toxin receptor (hHB-EGF) exclusively in salivary glands were generated. They reduced salivary secretion caused by diphtheria toxin injection, and apoptotic cell death was confirmed. Diphtheria toxin administration to genetically modified mice caused salivary gland-specific apoptosis and reduced saliva secretion. In addition, a stromal vascular fraction (a cell population rich in adipose-derived stem cells) was isolated from C57BL/6 mice, the original strain of the MUC7p-TRECK mice. In the process, it was suggested that the stromal vascular fraction exhibited severe cellular senescence along with passaging cultures.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10007_description_14":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"・2023(令和5)年度 科学研究費補助金 基盤研究(C) 研究成果報告書","subitem_description_language":"ja","subitem_description_type":"Other"},{"subitem_description":"・研究期間 (年度)：2020-04-01 – 2024-03-31","subitem_description_language":"ja","subitem_description_type":"Other"},{"subitem_description":"・研究分野：病理学","subitem_description_language":"ja","subitem_description_type":"Other"},{"subitem_description":"・研究成果の学術的意義や社会的意義 : 従来の主な唾液腺組織傷害モデルマウスは自己免疫疾患を発症し全身の様々な臓器・組織傷害が生じるため、唾液腺組織の傷害、修復・再生を詳細に検討するためには、唾液腺特異的な細胞・組織傷害モデルが求められていた。本研究で作出された遺伝子改変マウスは唾液腺特異的にアポトーシスによる細胞死が誘導されることが確認され、有用な唾液腺組織傷害や修復・再生のモデル動物になり得ると期待される。また脂肪幹細胞による唾液腺再生医療を実現するため、マウス鼠径部由来の脂肪幹細胞を多く含む細胞群を培養したところ、その初期に細胞老化が生じることが示唆され、細胞老化の制御により良好な脂肪幹細胞移植が達成できると示唆された。","subitem_description_language":"ja","subitem_description_type":"Other"}]},"item_10007_description_9":{"attribute_name":"研究課題番号","attribute_value_mlt":[{"subitem_description":"研究課題/領域番号 : 20K10002","subitem_description_language":"ja","subitem_description_type":"Other"}]},"item_10007_relation_17":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-20K10002/","subitem_relation_type_select":"URI"}}]},"item_10007_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2025-05-13"}],"displaytype":"detail","filename":"20K10002.pdf","filesize":[{"value":"309.8 KB"}],"format":"application/pdf","licensetype":"license_0","mimetype":"application/pdf","url":{"label":"20K10002","url":"https://tsurumi-u.repo.nii.ac.jp/record/2000189/files/20K10002.pdf"},"version_id":"ec895998-6d73-4c2f-986f-00c4ba0e4d0f"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"脂肪由来幹細胞","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"口腔乾燥症","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"再生医療","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"唾液腺","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"組織傷害","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"間質血管細胞群","subitem_subject_language":"ja","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_researcher":{"attribute_name":"研究代表者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"MATSUMOTO, Naoyuki","creatorNameLang":"en"},{"creatorName":"松本, 直行","creatorNameLang":"ja"},{"creatorName":"マツモト, ナオユキ","creatorNameLang":"ja-Kana"}],"familyNames":[{"familyName":"MATSUMOTO","familyNameLang":"en"},{"familyName":"松本","familyNameLang":"ja"},{"familyName":"マツモト","familyNameLang":"ja-Kana"}],"givenNames":[{"givenName":"Naoyuki","givenNameLang":"en"},{"givenName":"直行","givenNameLang":"ja"},{"givenName":"ナオユキ","givenNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"772","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"20386080","nameIdentifierScheme":"e-Rad_Researcher","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=20386080"},{"nameIdentifier":"772","nameIdentifierScheme":"WEKO著者ID"}]}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"脂肪由来幹細胞による唾液腺特異的傷害モデルマウスの組織再生と促進因子の網羅的解析","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"脂肪由来幹細胞による唾液腺特異的傷害モデルマウスの組織再生と促進因子の網羅的解析","subitem_title_language":"ja"},{"subitem_title":"Comprehensive analysis of tissue regeneration and promoting factors in a mouse model of salivary gland-specific injury by adipose-derived stem cells.","subitem_title_language":"en"}]},"item_type_id":"10007","owner":"10","path":["1744946052441"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2025-05-13"},"publish_date":"2025-05-13","publish_status":"0","recid":"2000189","relation_version_is_last":true,"title":["脂肪由来幹細胞による唾液腺特異的傷害モデルマウスの組織再生と促進因子の網羅的解析"],"weko_creator_id":"10","weko_shared_id":-1},"updated":"2025-05-13T02:54:34.114654+00:00"}