{"created":"2024-08-09T05:18:53.020300+00:00","id":2000116,"links":{},"metadata":{"_buckets":{"deposit":"37bc0163-033e-4000-96ad-728977622226"},"_deposit":{"created_by":10,"id":"2000116","owners":[10],"pid":{"revision_id":0,"type":"depid","value":"2000116"},"status":"published"},"_oai":{"id":"oai:tsurumi-u.repo.nii.ac.jp:02000116","sets":["100:101:103:1722471874793"]},"author_link":["951"],"item_10007_date_8":{"attribute_name":"報告年度","attribute_value_mlt":[{"subitem_date_issued_datetime":"2023-03-31","subitem_date_issued_type":"Issued"}]},"item_10007_description_13":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"研究成果の概要（和文）：本研究では歯髄組織片を多孔性メンブレンで挟み、培養したところ、組織辺縁への細胞集積が確認できた。また、細胞遊走のメカニズムに関して我々はSDF-１(stromal derived factor-1)に着目し、組織片の免疫染色を行い、72時間培養した組織片でSDF-1が最も強く発現していることが確認できた。さらにSDF-1の中和抗体を用いて培養を行ったところ、組織片への細胞集積が遅れることも確認できた。さらに他の因子として、歯髄組織片よりタンパクを抽出し、マルチプレックスを用いて解析を行ったところ、GRO-α、MCP-１の2つの因子が経時的に発現量が増加していることが確認できた。","subitem_description_language":"ja","subitem_description_type":"Abstract"},{"subitem_description":"研究成果の概要（英文）：In our study, dental pulp tissue fragments were placed between porous membranes and cultured, and cell migration at the tissue margins was observed. Next, we focused on SDF-1 (stromal derived factor-1) as a mechanism of cell migration, and immunostaining of the tissue fragments showed that SDF-1 was most strongly expressed in the tissue fragments cultured for 72 hours. Furthermore, when the tissue fragments were cultured with a neutralizing antibody against SDF-1, it was confirmed that the migration of cells in the tissue fragments was delayed. In addition, when proteins were extracted from the pulp tissue fragments and analyzed using multiplex assay, it was confirmed that the expression levels of two other factors, GRO-α and MCP-1, increased over time.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10007_description_14":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"・2022(令和4)年度 科学研究費補助金 若手研究 研究成果報告書","subitem_description_language":"ja","subitem_description_type":"Other"},{"subitem_description":"・研究期間 (年度)：2018-04-01 – 2023-03-31","subitem_description_language":"ja","subitem_description_type":"Other"},{"subitem_description":"・研究分野：再生医療","subitem_description_language":"ja","subitem_description_type":"Other"},{"subitem_description":"・研究成果の学術的意義や社会的意義 : 本研究の結果より、多孔性メンブレンで抜去歯より採取した歯髄組織片を挟むことで、輸送中も培養が可能となり、一般開業医などで採取後に、CPC(細胞培養加工施設)などへ輸送することによりオーダーメイドの再生医療の裾野の拡大が期待できる。また、細胞遊走のメカニズムに関してもSDF-１(stromal derived factor-1)、GRO-α、MCP-１の関連が示唆された。","subitem_description_language":"ja","subitem_description_type":"Other"}]},"item_10007_description_9":{"attribute_name":"研究課題番号","attribute_value_mlt":[{"subitem_description":"研究課題/領域番号 : 18K17183","subitem_description_language":"ja","subitem_description_type":"Other"}]},"item_10007_relation_17":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-18K17183/","subitem_relation_type_select":"URI"}}]},"item_10007_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2024-08-26"}],"displaytype":"detail","filename":"18K17183.pdf","filesize":[{"value":"243.0 kB"}],"format":"application/pdf","licensetype":"license_6","mimetype":"application/pdf","url":{"label":"18K17183","url":"https://tsurumi-u.repo.nii.ac.jp/record/2000116/files/18K17183.pdf"},"version_id":"bdefa292-c7d1-40a6-a008-ca6d945148b3"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"歯髄幹細胞","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"歯髄組織","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"凍結保存","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"間葉系幹細胞","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"再生医療","subitem_subject_language":"ja","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_researcher":{"attribute_name":"研究代表者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"TAKEBE, Yusuke","creatorNameLang":"en"},{"creatorName":"竹部, 祐生亮","creatorNameLang":"ja"},{"creatorName":"タケベ, ユウスケ","creatorNameLang":"ja-Kana"}],"familyNames":[{"familyName":"TAKEBE","familyNameLang":"en"},{"familyName":"竹部","familyNameLang":"ja"},{"familyName":"タケベ","familyNameLang":"ja-Kana"}],"givenNames":[{"givenName":"Yusuke","givenNameLang":"en"},{"givenName":"祐生亮","givenNameLang":"ja"},{"givenName":"ユウスケ","givenNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"951","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"50807097","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=50807097"},{"nameIdentifier":"951","nameIdentifierScheme":"WEKO著者ID"}]}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"歯髄幹細胞の効率的回収と輸送中の歯髄組織培養を可能とする新規凍結保存法の開発","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"歯髄幹細胞の効率的回収と輸送中の歯髄組織培養を可能とする新規凍結保存法の開発","subitem_title_language":"ja"},{"subitem_title":"Novel cryopreservation method for the effective collection of dental pulp stem cells and culturing dental pulp tissue over transport","subitem_title_language":"en"}]},"item_type_id":"10007","owner":"10","path":["1722471874793"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2024-08-26"},"publish_date":"2024-08-26","publish_status":"0","recid":"2000116","relation_version_is_last":true,"title":["歯髄幹細胞の効率的回収と輸送中の歯髄組織培養を可能とする新規凍結保存法の開発"],"weko_creator_id":"10","weko_shared_id":-1},"updated":"2024-09-02T07:53:16.228967+00:00"}