{"created":"2023-05-15T08:56:00.057258+00:00","id":1248,"links":{},"metadata":{"_buckets":{"deposit":"c2fdfa66-50b2-4c87-8f71-5c123ff66b45"},"_deposit":{"created_by":10,"id":"1248","owners":[10],"pid":{"revision_id":0,"type":"depid","value":"1248"},"status":"published"},"_oai":{"id":"oai:tsurumi-u.repo.nii.ac.jp:00001248","sets":["100:101:103:184"]},"author_link":["749"],"item_10007_date_8":{"attribute_name":"報告年度","attribute_value_mlt":[{"subitem_date_issued_datetime":"2014-03-31","subitem_date_issued_type":"Issued"}]},"item_10007_description_13":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"研究成果の概要（和文）：本研究は象牙質の主要非コラーゲン性タンパク質である象牙質シアロタンパク質(DSP)および象牙質リンタンパク質(DPP)が細胞分化誘導因子としての生理活性機能を有しているかを調べることを目的とした。ブタ象牙質より分離精製したDSPおよびDPP自体には生理活性作用はなく、TGF-b1と結合した微量のDSPおよびDPPが存在することが判明した。リコンビナントTGF-b1を用いた主要象牙質タンパク質に対するin vitro結合実験では、TGF-b1はコラーゲンにはほとんど結合しなかったが、DSPおよびDPPに結合することでその活性が維持されることが判明した。","subitem_description_language":"ja","subitem_description_type":"Abstract"},{"subitem_description":"研究成果の概要（英文）：We fractionated DPP and DSP along with TGF-b-like activity by ion exchange (IE) chromatography from developing pig molars and measured their alkaline phosphatase (ALP) stimulating activity in human periodontal (HPDL) cells with or without TGF-b receptor inhibitor. We then purified TGF-b-unbound or -bound DPP and DSP by reverse phase high performance liquid chromatography (RP-HPLC) using ALP-HPDL system. The TGF-b isoform bound to DPP and DSP was identified as being TGF-b1 by ELISA and LC-MS/MS analysis. We incubated carrier-free human recombinant TGF-b1 (CF-hTGF-b1) with TGF-b-unbound DPP or DSP and characterized the binding on IE-HPLC using the ALP-HPDL system. DPP and DSP rescued the loss of TGF-b1 activity. Approximately 19% and 10% of the ALP stimulating activities were retained by the binding of TGF-b1 to DPP and DSP, respectively. The type I collagen infrequently bound to CF-hTGFb1. We conclude that both DPP and DSP help retain TGF-b1 activity in porcine dentin.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10007_description_14":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"・2013(平成25)年度 科学研究費補助金 研究活動スタート支援 研究成果報告書","subitem_description_language":"ja","subitem_description_type":"Other"},{"subitem_description":"・研究期間 (年度)：2012-08-31 – 2014-03-31","subitem_description_language":"ja","subitem_description_type":"Other"},{"subitem_description":"・研究分野：医歯薬学","subitem_description_language":"ja","subitem_description_type":"Other"}]},"item_10007_description_9":{"attribute_name":"研究課題番号","attribute_value_mlt":[{"subitem_description":"研究課題/領域番号 : 24890265","subitem_description_language":"ja","subitem_description_type":"Other"}]},"item_10007_relation_17":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-24890265/","subitem_relation_type_select":"URI"}}]},"item_10007_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-05-09"}],"displaytype":"detail","filename":"24890265.pdf","filesize":[{"value":"294.7 kB"}],"format":"application/pdf","licensetype":"license_6","mimetype":"application/pdf","url":{"label":"24890265","url":"https://tsurumi-u.repo.nii.ac.jp/record/1248/files/24890265.pdf"},"version_id":"e4d0a39e-35a4-4899-ad42-604eff5b95c6"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"歯学","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"再生医学","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"細胞・組織","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"タンパク質","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"生理活性物質","subitem_subject_language":"ja","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_researcher":{"attribute_name":"研究代表者","attribute_type":"creator","attribute_value_mlt":[{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"","affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"http://www.isni.org/isni/"}],"affiliationNames":[{"affiliationName":"","affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"YAMAKOSHI, Yasuo","creatorNameLang":"en"},{"creatorName":"山越, 康雄","creatorNameLang":"ja"},{"creatorName":"ヤマコシ, ヤスオ","creatorNameLang":"ja-Kana"}],"familyNames":[{"familyName":"YAMAKOSHI","familyNameLang":"en"},{"familyName":"山越","familyNameLang":"ja"},{"familyName":"ヤマコシ","familyNameLang":"ja-Kana"}],"givenNames":[{"givenName":"Yasuo","givenNameLang":"en"},{"givenName":"康雄","givenNameLang":"ja"},{"givenName":"ヤスオ","givenNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"749","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"20182470","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=20182470"},{"nameIdentifier":"749","nameIdentifierScheme":"WEKO著者ID"}]}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"ブタ歯髄組織中の象牙質シアロリンタンパク質の構造機能解明と臨床応用への基礎研究","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"ブタ歯髄組織中の象牙質シアロリンタンパク質の構造機能解明と臨床応用への基礎研究","subitem_title_language":"ja"},{"subitem_title":"Elucidation of structural function and basic research for clinical application of dentin sialophosphoprotein in porcine pulp","subitem_title_language":"en"}]},"item_type_id":"10007","owner":"10","path":["184"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2022-05-09"},"publish_date":"2022-05-09","publish_status":"0","recid":"1248","relation_version_is_last":true,"title":["ブタ歯髄組織中の象牙質シアロリンタンパク質の構造機能解明と臨床応用への基礎研究"],"weko_creator_id":"10","weko_shared_id":-1},"updated":"2024-08-01T07:44:55.513912+00:00"}