{"created":"2023-05-15T08:55:56.916997+00:00","id":1189,"links":{},"metadata":{"_buckets":{"deposit":"c0b40910-7b4f-4895-922c-03fa6a219a2e"},"_deposit":{"created_by":10,"id":"1189","owners":[10],"pid":{"revision_id":0,"type":"depid","value":"1189"},"status":"published"},"_oai":{"id":"oai:tsurumi-u.repo.nii.ac.jp:00001189","sets":["100:101:103:178"]},"author_link":["770"],"item_10007_date_8":{"attribute_name":"報告年度","attribute_value_mlt":[{"subitem_date_issued_datetime":"2016-03-31","subitem_date_issued_type":"Issued"}]},"item_10007_description_13":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"研究成果の概要（和文）：本研究では、ヒト歯髄組織から多分化能を有するMuse（Multilineage-differentiating stress enduring）細胞を分離・培養する方法を確立するために研究を行った。Muse細胞に関するプロトコールに準じて染色後、セルソータにてソーティングを行い、クラスタ形成された細胞のみをピックアップして培養し、自己複製能を確認した。また、三胚葉分化能についてRT-PCRで確認した。以上のことから、ヒト歯髄からMuse細胞を分離できることが明らかとなった。","subitem_description_language":"ja","subitem_description_type":"Abstract"},{"subitem_description":"研究成果の概要（英文）：In this research, we tried to establish the method of isolate and culture Muse (Multilineage-differentiating stress enduring) cells from human dental pulp tissue. Three strains of cultured human dental pulp cells were used. The methods of cell culture, cell sorting, and cluster formation, according to the protocol. In addition, pluripotency were confirmed by RT-PCR. It became clear that can be separated the Muse cells from the human dental pulp cells.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10007_description_14":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"・2015(平成27)年度 科学研究費補助金 若手研究(B) 研究成果報告書","subitem_description_language":"ja","subitem_description_type":"Other"},{"subitem_description":"・研究期間 (年度)：2013-04-01 – 2016-03-31","subitem_description_language":"ja","subitem_description_type":"Other"},{"subitem_description":"・研究分野：歯周病学","subitem_description_language":"ja","subitem_description_type":"Other"}]},"item_10007_description_9":{"attribute_name":"研究課題番号","attribute_value_mlt":[{"subitem_description":"研究課題/領域番号 : 25862061","subitem_description_language":"ja","subitem_description_type":"Other"}]},"item_10007_relation_17":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-25862061/","subitem_relation_type_select":"URI"}}]},"item_10007_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-04-20"}],"displaytype":"detail","filename":"25862061.pdf","filesize":[{"value":"119.5 kB"}],"format":"application/pdf","licensetype":"license_6","mimetype":"application/pdf","url":{"label":"25862061","url":"https://tsurumi-u.repo.nii.ac.jp/record/1189/files/25862061.pdf"},"version_id":"76d556e6-2fb5-44b4-9f13-ef12fa1b250b"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Muse細胞","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"歯髄","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"歯周組織再生","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"自己複製能","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"三胚葉分化能","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"SSEA-3","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"CD105","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_researcher":{"attribute_name":"研究代表者","attribute_type":"creator","attribute_value_mlt":[{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"","affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"http://www.isni.org/isni/"}],"affiliationNames":[{"affiliationName":"","affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"NAGANO, Takatoshi","creatorNameLang":"en"},{"creatorName":"長野, 孝俊","creatorNameLang":"ja"},{"creatorName":"ナガノ, タカトシ","creatorNameLang":"ja-Kana"}],"familyNames":[{"familyName":"NAGANO","familyNameLang":"en"},{"familyName":"長野","familyNameLang":"ja"},{"familyName":"ナガノ","familyNameLang":"ja-Kana"}],"givenNames":[{"givenName":"Takatoshi","givenNameLang":"en"},{"givenName":"孝俊","givenNameLang":"ja"},{"givenName":"タカトシ","givenNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"770","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"10386914","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=10386914"},{"nameIdentifier":"770","nameIdentifierScheme":"WEKO著者ID"}]}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"歯髄由来Muse細胞を用いた新規組織工学的歯周組織再生療法の開発","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"歯髄由来Muse細胞を用いた新規組織工学的歯周組織再生療法の開発","subitem_title_language":"ja"},{"subitem_title":"Development of new pediodontal regeneration therapy with Muse cells from dental pulp","subitem_title_language":"en"}]},"item_type_id":"10007","owner":"10","path":["178"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2022-04-20"},"publish_date":"2022-04-20","publish_status":"0","recid":"1189","relation_version_is_last":true,"title":["歯髄由来Muse細胞を用いた新規組織工学的歯周組織再生療法の開発"],"weko_creator_id":"10","weko_shared_id":-1},"updated":"2024-08-01T08:37:22.467556+00:00"}