{"created":"2023-05-15T08:55:56.643989+00:00","id":1183,"links":{},"metadata":{"_buckets":{"deposit":"bade49e2-f4c2-4081-a793-b3b756a48c05"},"_deposit":{"created_by":10,"id":"1183","owners":[10],"pid":{"revision_id":0,"type":"depid","value":"1183"},"status":"published"},"_oai":{"id":"oai:tsurumi-u.repo.nii.ac.jp:00001183","sets":["100:101:103:178"]},"author_link":["886"],"item_10007_date_8":{"attribute_name":"報告年度","attribute_value_mlt":[{"subitem_date_issued_datetime":"2016-03-31","subitem_date_issued_type":"Issued"}]},"item_10007_description_13":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"研究成果の概要（和文）：歯周組織の歯根膜非コラーゲン性タンパク質（NCP）の分離精製、性質を調べることを目的とし、特に初期破壊に関連する110 kDa Stains-all色素陽性のタンパク質（SAPP)に注目した。ブタ乳切歯歯根膜から各種溶液で歯根膜NCPを段階的に抽出して試料とした。110kDa SAPPはマス分析と免疫科学でデコリンであると同定した。へパリンカラムで精製したデコリンの画分には、hPDL細胞の細胞培養法とイライザ法でTGF-b1が存在することがわかった。これにより、炎症性変化で好中球エラスターゼがデコリンを分解すると、それに結合しているTGF-b１が遊離し、組織の修復に働くと考えられた。","subitem_description_language":"ja","subitem_description_type":"Abstract"},{"subitem_description":"研究成果の概要（英文）：It is shown that the early destruction feature of periodontal ligament (PDL) on periodontal disease is the degradation of the non-collagenous proteins (NCP) covered collagen bundles. The aim is the characterization of 110kDa Stains-all positive protein (SAPP) belonged to NCP, which is degraded first by the neutrophil elastase increased in the inflamed PDL. The 110kDa SAPP was detected by Stains-all staining after SDS electrophoresis in the NCP extracted from porcine mandibles' deciduous incisor tooth PDL and identified to be decorin by MASS and immunochemical determination. The decorin purified by heparin affinity column was determined to bind the TGF-b1 by the cell culture system of hPDL cell and ELISA. On the periodontal disease, it is suggested that the degradation of decorin bound to the collagen bundles by the action of the neutrophil elastase releases the TGF-b 1 to reconstruct the injured PDL.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10007_description_14":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"・2015(平成27)年度 科学研究費補助金 基盤研究(C) 研究成果報告書","subitem_description_language":"ja","subitem_description_type":"Other"},{"subitem_description":"・研究期間 (年度)：2013-04-01 – 2016-03-31","subitem_description_language":"ja","subitem_description_type":"Other"},{"subitem_description":"・研究分野：医歯薬","subitem_description_language":"ja","subitem_description_type":"Other"}]},"item_10007_description_9":{"attribute_name":"研究課題番号","attribute_value_mlt":[{"subitem_description":"研究課題/領域番号 : 25463231","subitem_description_language":"ja","subitem_description_type":"Other"}]},"item_10007_relation_17":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-25463231/","subitem_relation_type_select":"URI"}}]},"item_10007_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-04-20"}],"displaytype":"detail","filename":"25463231.pdf","filesize":[{"value":"436.2 kB"}],"format":"application/pdf","licensetype":"license_6","mimetype":"application/pdf","url":{"label":"25463231","url":"https://tsurumi-u.repo.nii.ac.jp/record/1183/files/25463231.pdf"},"version_id":"e2157550-4b42-4ad8-832a-42797f8fc63d"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"歯周病","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"歯根膜","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"好中球エラスターゼ","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"デコリン","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"トランスフォーミング成長因子ベータ","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"非コラーゲン性タンパク質","subitem_subject_language":"ja","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_researcher":{"attribute_name":"研究代表者","attribute_type":"creator","attribute_value_mlt":[{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"","affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"http://www.isni.org/isni/"}],"affiliationNames":[{"affiliationName":"","affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"FUKAE, Makoto","creatorNameLang":"en"},{"creatorName":"深江, 允","creatorNameLang":"ja"},{"creatorName":"フカエ, マコト","creatorNameLang":"ja-Kana"}],"familyNames":[{"familyName":"FUKAE","familyNameLang":"en"},{"familyName":"深江","familyNameLang":"ja"},{"familyName":"フカエ","familyNameLang":"ja-Kana"}],"givenNames":[{"givenName":"Makoto","givenNameLang":"en"},{"givenName":"允","givenNameLang":"ja"},{"givenName":"マコト","givenNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"886","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"40064373","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=40064373"},{"nameIdentifier":"886","nameIdentifierScheme":"WEKO著者ID"}]}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"歯根膜の初期破壊に関わる非コラーゲン性構造タンパク質について","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"歯根膜の初期破壊に関わる非コラーゲン性構造タンパク質について","subitem_title_language":"ja"},{"subitem_title":"Non-collagenous proteins involved in an early destruction of periodontal ligament","subitem_title_language":"en"}]},"item_type_id":"10007","owner":"10","path":["178"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2022-04-20"},"publish_date":"2022-04-20","publish_status":"0","recid":"1183","relation_version_is_last":true,"title":["歯根膜の初期破壊に関わる非コラーゲン性構造タンパク質について"],"weko_creator_id":"10","weko_shared_id":-1},"updated":"2024-08-02T03:03:29.648155+00:00"}