{"created":"2023-05-15T08:55:56.471799+00:00","id":1180,"links":{},"metadata":{"_buckets":{"deposit":"91c1df82-228c-4683-98ba-97da102e8a9d"},"_deposit":{"created_by":10,"id":"1180","owners":[10],"pid":{"revision_id":0,"type":"depid","value":"1180"},"status":"published"},"_oai":{"id":"oai:tsurumi-u.repo.nii.ac.jp:00001180","sets":["100:101:103:178"]},"author_link":["883"],"item_10007_date_8":{"attribute_name":"報告年度","attribute_value_mlt":[{"subitem_date_issued_datetime":"2016-03-31","subitem_date_issued_type":"Issued"}]},"item_10007_description_13":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"研究成果の概要（和文）：ポリADP-リボシル化反応は、ポリADP-リボース合成酵素（PARP）によって行われ、細胞内の様々な作用に関与している。PARPの間葉系幹細胞（MSC）の分化への関与も報告があるが、即ちPARP阻害剤のMSCへの副作用の可能性を意味する。本研究ではPARP阻害剤のMSCへの影響を調べた。1 μM PJ34の添加で骨芽細胞への分化が抑制されたが、軟骨や脂肪への分化には影響がなかった。mRNAレベルの骨分化マーカーや転写因子の発現は、PJ34の投与で低下した。よって、PARP阻害剤は骨芽細胞分化を抑制し、ポリADP-リボシル化反応がBMP-2経路を調整することによって関与することが示唆された。","subitem_description_language":"ja","subitem_description_type":"Abstract"},{"subitem_description":"研究成果の概要（英文）：Poly(ADP-ribosyl)ation is known to be involved in a variety of cellular processes by poly(ADP-ribose) polymerase (PARP). PARP involvement in MSCs (Mesenchymal Stem Cells) differentiation has been reported suggesting possible side effects of PARP inhibitors on MSCs. Therefore, effects of PARP inhibitors on MSCs were examined in this study. MSCs showed suppressed osteogenic differentiation after 1 μM PJ34 treatment without showing cytotoxicity, while differentiation of MSCs into chondrocyte or adipocyte was not affected. The mRNA induction of osteogenic markers such as and regulators of transcription factors was suppressed by PJ34 treatment. Extracellular mineralized matrix formation was also suppressed. These results strongly suggest that PARP inhibitors would suppress osteogenic differentiation and poly(ADP-ribosyl)ation may play a physiological role in osteogenic differentiation by regulating BMP-2 signaling pathway.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10007_description_14":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"・2015(平成27)年度 科学研究費補助金 基盤研究(C) 研究成果報告書","subitem_description_language":"ja","subitem_description_type":"Other"},{"subitem_description":"・研究期間 (年度)：2013-04-01 – 2016-03-31","subitem_description_language":"ja","subitem_description_type":"Other"},{"subitem_description":"・研究分野：口腔外科","subitem_description_language":"ja","subitem_description_type":"Other"}]},"item_10007_description_9":{"attribute_name":"研究課題番号","attribute_value_mlt":[{"subitem_description":"研究課題/領域番号 : 25463157","subitem_description_language":"ja","subitem_description_type":"Other"}]},"item_10007_relation_17":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-25463157/","subitem_relation_type_select":"URI"}}]},"item_10007_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-04-20"}],"displaytype":"detail","filename":"25463157.pdf","filesize":[{"value":"557.8 kB"}],"format":"application/pdf","licensetype":"license_6","mimetype":"application/pdf","url":{"label":"25463157","url":"https://tsurumi-u.repo.nii.ac.jp/record/1180/files/25463157.pdf"},"version_id":"e7797fa2-8cb3-47c0-a4cb-6679afd7298a"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"間葉系幹細胞","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"骨芽細胞","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"ポリADP-リボシル化","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"分化","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"骨造成","subitem_subject_language":"ja","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_researcher":{"attribute_name":"研究代表者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"川口, 浩司","creatorNameLang":"ja"},{"creatorName":"KAWAGUCHI, Kouji","creatorNameLang":"en"}],"nameIdentifiers":[{},{},{}]}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"骨造成促進のための新規治療法開発に向けた基礎的研究","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"骨造成促進のための新規治療法開発に向けた基礎的研究","subitem_title_language":"ja"},{"subitem_title":"Basic research for establishment of new therapy for acceralation of bone augumentation","subitem_title_language":"en"}]},"item_type_id":"10007","owner":"10","path":["178"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2022-04-20"},"publish_date":"2022-04-20","publish_status":"0","recid":"1180","relation_version_is_last":true,"title":["骨造成促進のための新規治療法開発に向けた基礎的研究"],"weko_creator_id":"10","weko_shared_id":-1},"updated":"2024-02-01T02:29:38.604644+00:00"}