{"created":"2023-05-15T08:55:54.405248+00:00","id":1141,"links":{},"metadata":{"_buckets":{"deposit":"19e0a659-5532-4eed-b03d-d007616a696a"},"_deposit":{"created_by":10,"id":"1141","owners":[10],"pid":{"revision_id":0,"type":"depid","value":"1141"},"status":"published"},"_oai":{"id":"oai:tsurumi-u.repo.nii.ac.jp:00001141","sets":["100:101:103:177"]},"author_link":["852"],"item_10007_date_8":{"attribute_name":"報告年度","attribute_value_mlt":[{"subitem_date_issued_datetime":"2017-03-31","subitem_date_issued_type":"Issued"}]},"item_10007_description_13":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"研究成果の概要（和文）：Enthesisは、腱と骨の間に線維軟骨が介在する線維軟骨性付着とこれらが介在しない線維性付着に分類される。このうち線維軟骨性付着部では、生後7週以降、野生型に比べてAnxa5機能欠損マウスで特異的な皮質骨面積と線維軟骨層の増大を認めた。軟骨層には野生型と比べてアルカリフォスファターゼ活性の強い細胞がより多く観察された。一方、線維性付着部ではAnxa5欠損による影響を受けなかった。siRNAによってATDC5のAnxa5遺伝子をノックダウンすると軟骨分化マーカーの遺伝子発現が増大した。これらの結果は、Anxa5が腱・靭帯付着部の線維軟骨の分化と石灰化を負に制御することを示唆する。","subitem_description_language":"ja","subitem_description_type":"Abstract"},{"subitem_description":"研究成果の概要（英文）：Entheses can be further divided to fibrocartilaginous and fibrous entheses according to the type of tissue present at the skeletal attachment site. In Anxa5-deficient (Anxa5-/-) mice, the sizes of bone ridge outgrowths at the fibrocartilaginous entheses of tibiae and femur were increased after 7 weeks of age. This bone overgrowth was not observed at the fibrous enthesis where Anxa5-lacZ is hardly expressed. At the fibrocartilaginous entheses, more ALP-expressing cells were observed in the fibrocartilage layer outside of the bone in the mutant mice. Then, to examine the role of Anxa5 on cartilage differentiation, we performed Anxa5 gene knockdown in vitro using siRNA. Depletion of endogenous Anxa5 with siRNA increased mRNA expressions of differentiated marker of chondrocyte. Together, these results suggest that Anxa5 prevents bone overgrowth at the fibrocartilaginous entheses.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10007_description_14":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"・2016(平成28)年度 科学研究費補助金 基盤研究(C) 研究成果報告書","subitem_description_language":"ja","subitem_description_type":"Other"},{"subitem_description":"・研究期間 (年度)：2014-04-01 – 2017-03-31","subitem_description_language":"ja","subitem_description_type":"Other"},{"subitem_description":"・研究分野：歯科学","subitem_description_language":"ja","subitem_description_type":"Other"}]},"item_10007_description_9":{"attribute_name":"研究課題番号","attribute_value_mlt":[{"subitem_description":"研究課題/領域番号 : 26462825","subitem_description_language":"ja","subitem_description_type":"Other"}]},"item_10007_relation_17":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-26462825/","subitem_relation_type_select":"URI"}}]},"item_10007_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-04-19"}],"displaytype":"detail","filename":"26462825.pdf","filesize":[{"value":"189.7 kB"}],"format":"application/pdf","licensetype":"license_6","mimetype":"application/pdf","url":{"label":"26462825","url":"https://tsurumi-u.repo.nii.ac.jp/record/1141/files/26462825.pdf"},"version_id":"d09fb249-7aba-42cd-bd2a-56f09cbe3cbd"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Annexin a5","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"腱","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"靭帯","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"骨","subitem_subject_language":"ja","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_researcher":{"attribute_name":"研究代表者","attribute_type":"creator","attribute_value_mlt":[{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"","affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"http://www.isni.org/isni/"}],"affiliationNames":[{"affiliationName":"","affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"SHIMADA, Akemi","creatorNameLang":"en"},{"creatorName":"島田, 明美","creatorNameLang":"ja"},{"creatorName":"シマダ, アケミ","creatorNameLang":"ja-Kana"}],"familyNames":[{"familyName":"SHIMADA","familyNameLang":"en"},{"familyName":"島田","familyNameLang":"ja"},{"familyName":"シマダ","familyNameLang":"ja-Kana"}],"givenNames":[{"givenName":"Akemi","givenNameLang":"en"},{"givenName":"明美","givenNameLang":"ja"},{"givenName":"アケミ","givenNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"852","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"00339813","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=00339813"},{"nameIdentifier":"852","nameIdentifierScheme":"WEKO著者ID"}]}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"アネキシンA5による腱・靭帯と骨付着部(enthesis)のリモデリングの制御","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"アネキシンA5による腱・靭帯と骨付着部(enthesis)のリモデリングの制御","subitem_title_language":"ja"},{"subitem_title":"Regulation of bone remodeling by Annexin A5 at tendon/ligament insertion sites","subitem_title_language":"en"}]},"item_type_id":"10007","owner":"10","path":["177"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2022-04-19"},"publish_date":"2022-04-19","publish_status":"0","recid":"1141","relation_version_is_last":true,"title":["アネキシンA5による腱・靭帯と骨付着部(enthesis)のリモデリングの制御"],"weko_creator_id":"10","weko_shared_id":-1},"updated":"2024-08-02T01:30:28.254299+00:00"}