{"created":"2023-05-15T08:55:49.878619+00:00","id":1059,"links":{},"metadata":{"_buckets":{"deposit":"dbe4fcad-4266-494a-81c9-ec6640e28750"},"_deposit":{"created_by":10,"id":"1059","owners":[10],"pid":{"revision_id":0,"type":"depid","value":"1059"},"status":"published"},"_oai":{"id":"oai:tsurumi-u.repo.nii.ac.jp:00001059","sets":["100:101:103:160"]},"author_link":["805"],"item_10007_date_8":{"attribute_name":"報告年度","attribute_value_mlt":[{"subitem_date_issued_datetime":"2019-03-31","subitem_date_issued_type":"Issued"}]},"item_10007_description_13":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"研究成果の概要（和文）：BM-CMを増殖期、コンフルエント期、石灰化期の３つの期間にわけてHUCPVCsに添加した。タイムポイントは１・２・３週としてALP染色、Alizarin Red染色、遺伝子発現（Runx2、Osteocalcin、Ⅰ型コラーゲン）、Ca量の測定を評価方法とした。 BM-CMの３つの期間の検討では、コンフルエント期は他の時期よりもALP染色、Alizarin Red染色での陽性染色反応が明らかであり、また遺伝子発現にて Runx2 やOsteocalcinもより強く発現していた。コンフルエント期のBM-CMを用いることにより、HUCPVCsをより効果的に石灰化誘導されることが示唆された。","subitem_description_language":"ja","subitem_description_type":"Abstract"},{"subitem_description":"研究成果の概要（英文）：We focused on Human umbilical cord perivascular cells (HUCPVCs) as a new cellular source.The objective of this study is to investigate bone differentiation induction of HUCPVCs based on the collection term of bone marrow cell’s condition medium. BM-CM was divided into three according to the collection time.（Proliferate, Confluent, Calcification）HUCPVCs were cultured with each period of BM-CM, and the state of differentiation was evaluated in RT-PCR, ALP staining and Alizarin staining. Confluent and Calcification groups for 2 weeks showed more expression of Runx 2 than other group. HUCPVCs which were cultured with Confluent BM-CM for 2 weeks showed positive staining with ALP staining and Alizarin staining than other groups. In the conventional method, HUCPVCs is required to culture for 3 weeks before calcification. The results suggested that HUCPVCs which are cultured using confluent BM - CM show calcification tendency in 2 weeks.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10007_description_14":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"・2018(平成30)年度 科学研究費補助金 若手研究(B) 研究成果報告書","subitem_description_language":"ja","subitem_description_type":"Other"},{"subitem_description":"・研究期間 (年度)：2016-04-01 – 2019-03-31","subitem_description_language":"ja","subitem_description_type":"Other"},{"subitem_description":"・研究分野：口腔再生医学","subitem_description_language":"ja","subitem_description_type":"Other"},{"subitem_description":"・研究成果の学術的意義や社会的意義 : ヒト臍帯動・ 静脈周囲に存在する間葉系幹細胞Human Umbilical Cord PeriVascular cellsは医療廃棄物となった臍帯より採取するためドナーに新たな侵襲を加えることなく十分な細胞を確保できるメリットがある。また、免疫寛容性を有することから今後他家移植への臨床応用細胞ソースとして期待されている。本研究では臨床応用を見据えて無血清培地を用いて、ヒト臍帯組織間葉系幹細胞をより効果的に骨系へと分化させるために、最適なBM-CM採取期間について検討した。新たな幹細胞ソースの生体移植細胞としての有用性について探り、新規の骨再生療法を開発することを目的とした。","subitem_description_language":"ja","subitem_description_type":"Other"}]},"item_10007_description_9":{"attribute_name":"研究課題番号","attribute_value_mlt":[{"subitem_description":"研究課題/領域番号 : 16K20547","subitem_description_language":"ja","subitem_description_type":"Other"}]},"item_10007_relation_17":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-16K20547/","subitem_relation_type_select":"URI"}}]},"item_10007_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-03-16"}],"displaytype":"detail","filename":"16K20547.pdf","filesize":[{"value":"158.1 kB"}],"format":"application/pdf","licensetype":"license_6","mimetype":"application/pdf","url":{"label":"16K20547","url":"https://tsurumi-u.repo.nii.ac.jp/record/1059/files/16K20547.pdf"},"version_id":"543f8cbb-d288-4c64-8523-7d9aa99fcba6"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"臍帯組織由来幹細胞","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"骨髄細胞培養上清","subitem_subject_language":"ja","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_researcher":{"attribute_name":"研究代表者","attribute_type":"creator","attribute_value_mlt":[{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"","affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"http://www.isni.org/isni/"}],"affiliationNames":[{"affiliationName":"","affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"KAJIYAMA, Sohtaro","creatorNameLang":"en"},{"creatorName":"梶山, 創太郎","creatorNameLang":"ja"},{"creatorName":"カジヤマ, ソウタロウ","creatorNameLang":"ja-Kana"}],"familyNames":[{"familyName":"KAJIYAMA","familyNameLang":"en"},{"familyName":"梶山","familyNameLang":"ja"},{"familyName":"カジヤマ","familyNameLang":"ja-Kana"}],"givenNames":[{"givenName":"Sohtaro","givenNameLang":"en"},{"givenName":"創太郎","givenNameLang":"ja"},{"givenName":"ソウタロウ","givenNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"805","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"00760414","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=00760414"},{"nameIdentifier":"805","nameIdentifierScheme":"WEKO著者ID"}]}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"無血清培地で分化・誘導したヒト臍帯組織由来幹細胞を用いた骨再生療法の開発","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"無血清培地で分化・誘導したヒト臍帯組織由来幹細胞を用いた骨再生療法の開発","subitem_title_language":"ja"},{"subitem_title":"Study of bone regeneration therapy using Human Umbilical Cord PeriVascular cells differentiated and induced in serum-free medium","subitem_title_language":"en"}]},"item_type_id":"10007","owner":"10","path":["160"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2022-03-16"},"publish_date":"2022-03-16","publish_status":"0","recid":"1059","relation_version_is_last":true,"title":["無血清培地で分化・誘導したヒト臍帯組織由来幹細胞を用いた骨再生療法の開発"],"weko_creator_id":"10","weko_shared_id":-1},"updated":"2024-08-01T23:54:59.880592+00:00"}