{"created":"2023-05-15T08:55:49.813036+00:00","id":1058,"links":{},"metadata":{"_buckets":{"deposit":"9d0d5d90-4acc-4222-ba3f-1af5688db57a"},"_deposit":{"created_by":10,"id":"1058","owners":[10],"pid":{"revision_id":0,"type":"depid","value":"1058"},"status":"published"},"_oai":{"id":"oai:tsurumi-u.repo.nii.ac.jp:00001058","sets":["100:101:103:160"]},"author_link":["804"],"item_10007_date_8":{"attribute_name":"報告年度","attribute_value_mlt":[{"subitem_date_issued_datetime":"2019-03-31","subitem_date_issued_type":"Issued"}]},"item_10007_description_13":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"研究成果の概要（和文）：SEM観察でGM-CSFは粗造で不規則な表面形態を生じ, MTTアッセイでは48時間後に細胞生存率に有意差が認められた. TNF-α分泌はGM-CSFなしで48時間後に24時間後と比較して有意に減少し, GM-CSFは24, 48時間後にTNF-αの分泌を有意に増加した. IL-4分泌は, 24時間, 48時間でGM-CSF刺激の有無に関わらず有意に異なった. GM-CSF刺激の24, 48時間後にIL-4分泌が有意に増加した.これらの結果は, Ti上で培養したマクロファージについてGM-CSFによって刺激すると抗炎症性および炎症性サイトカインの分泌を促進する可能性があることを示唆している.","subitem_description_language":"ja","subitem_description_type":"Abstract"},{"subitem_description":"研究成果の概要（英文）：We investigated the secretion of tumor necrosis factor-α (TNF-α) and interleukin-4 (IL-4) from mouse macrophages(RAW264.7) activated by GM-CSF. RAW264.7 cells were cultured on titanium (Ti) discs. Secretion of TNF-α and IL-4 was evaluated using ELISA at 24h and 48h. Cell morphologies were observed using SEM, and cell viability was accessed by an MTT assay. GM-CSF caused rough and irregular surface morphology on the macrophages and resulted in a significant difference in cell viability after 48h. TNF-α secretion significantly decreased after 48h without GM-CSF compared with that at 24h. GM-CSF significantly increased the secretion of TNF-α after 24h and 48h. IL-4 secretion was significantly different with or without GM-CSF stimulation at 24h and 48h. There was a significant increase in IL-4 secretion 24h and 48h after GM-CSF stimulation. These results suggest that macrophage stimulated GM-CSF may promote secretion of anti-inflammatory and pro-inflammatory cytokines on Ti.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10007_description_14":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"・2018(平成30)年度 科学研究費補助金 若手研究(B) 研究成果報告書","subitem_description_language":"ja","subitem_description_type":"Other"},{"subitem_description":"・研究期間 (年度)：2016-04-01 – 2019-03-31","subitem_description_language":"ja","subitem_description_type":"Other"},{"subitem_description":"・研究分野：生体材料学","subitem_description_language":"ja","subitem_description_type":"Other"},{"subitem_description":"・研究成果の学術的意義や社会的意義 : インプラント埋入後の創傷治癒は, 生活反応期, 創内浄化期, 組織修復期, 組織再構築期の４つのステージに分けられる. 各ステージにおいて働く細胞があり, 各細胞を活性化するサイトカインが存在する. 今回の研究では, 初期の治癒反応に関係するマクロファージとGM-CSFに狙いを絞って評価した. その結果, マクロファージをGM-CSFで刺激することにより創傷性サイトカインおよび炎症性サイトカインが促進された.今後これらのサイトカインを組み合わせてチタン表面へ固定化し各ステージで働く細胞活性を促進することにより創傷治癒を短縮できる可能性があると考えられる.","subitem_description_language":"ja","subitem_description_type":"Other"}]},"item_10007_description_9":{"attribute_name":"研究課題番号","attribute_value_mlt":[{"subitem_description":"研究課題/領域番号 : 16K20546","subitem_description_language":"ja","subitem_description_type":"Other"}]},"item_10007_relation_17":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-16K20546/","subitem_relation_type_select":"URI"}}]},"item_10007_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-03-16"}],"displaytype":"detail","filename":"16K20546.pdf","filesize":[{"value":"200.2 kB"}],"format":"application/pdf","licensetype":"license_6","mimetype":"application/pdf","url":{"label":"16K20546","url":"https://tsurumi-u.repo.nii.ac.jp/record/1058/files/16K20546.pdf"},"version_id":"65cf1de8-fa99-457b-b93a-243b0003680a"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"GM-CSF","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"マクロファージ","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"TNF-α","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"IL-4","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"チタン","subitem_subject_language":"ja","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_researcher":{"attribute_name":"研究代表者","attribute_type":"creator","attribute_value_mlt":[{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"","affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"http://www.isni.org/isni/"}],"affiliationNames":[{"affiliationName":"","affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"SUZUKI, Takuma","creatorNameLang":"en"},{"creatorName":"鈴木, 琢磨","creatorNameLang":"ja"},{"creatorName":"スズキ, タクマ","creatorNameLang":"ja-Kana"}],"familyNames":[{"familyName":"SUZUKI","familyNameLang":"en"},{"familyName":"鈴木","familyNameLang":"ja"},{"familyName":"スズキ","familyNameLang":"ja-Kana"}],"givenNames":[{"givenName":"Takuma","givenNameLang":"en"},{"givenName":"琢磨","givenNameLang":"ja"},{"givenName":"タクマ","givenNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"804","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"80739334","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=80739334"},{"nameIdentifier":"804","nameIdentifierScheme":"WEKO著者ID"}]}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"タンパク質固定化技術を用いた生体適合性インプラントの開発","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"タンパク質固定化技術を用いた生体適合性インプラントの開発","subitem_title_language":"ja"},{"subitem_title":"Development of biocompatible implant using protein immobilization method","subitem_title_language":"en"}]},"item_type_id":"10007","owner":"10","path":["160"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2022-03-16"},"publish_date":"2022-03-16","publish_status":"0","recid":"1058","relation_version_is_last":true,"title":["タンパク質固定化技術を用いた生体適合性インプラントの開発"],"weko_creator_id":"10","weko_shared_id":-1},"updated":"2024-08-01T23:45:15.399221+00:00"}