{"created":"2023-05-15T08:55:49.401297+00:00","id":1051,"links":{},"metadata":{"_buckets":{"deposit":"88488ab6-1ee3-4b02-9634-b51871b6b0ce"},"_deposit":{"created_by":10,"id":"1051","owners":[10],"pid":{"revision_id":0,"type":"depid","value":"1051"},"status":"published"},"_oai":{"id":"oai:tsurumi-u.repo.nii.ac.jp:00001051","sets":["100:101:103:160"]},"author_link":["797"],"item_10007_date_8":{"attribute_name":"報告年度","attribute_value_mlt":[{"subitem_date_issued_datetime":"2019-03-31","subitem_date_issued_type":"Issued"}]},"item_10007_description_13":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"研究成果の概要（和文）：本研究の目的は、エナメル質形成過程におけるTGF-βの役割について解明することである。マウス由来のエナメル上皮細胞（mHAT9d）を用いて行ったPCRより、TGF-βアイソフォームの添加によって成熟期エナメルタンパク質関連遺伝子の優位な発現上昇を認めた。さらに、TGF-βアイソフォームの添加でカスパーゼ－３およびDNA断片の有意な上昇が認められ、その分布には相同性が認められた。TGFは複雑な生物学的機能を働かせるためにアイソフォーム特異的な方法による調整能を有しており、細胞増殖、さらに成熟期でのエナメル芽細胞のアポトーシス誘導について調整している可能性を示唆した。","subitem_description_language":"ja","subitem_description_type":"Abstract"},{"subitem_description":"研究成果の概要（英文）：Objectives are to elucidate it about how role of TGF-β isoforms during enamel formation. We were using a dental epithelial cell line derived from the apical bud of a mouse incisor (mHAT9d cells). We prepared mRNAs from culture supernatant of mHAT9d for Quantitative PCR Analysis of three TGF-β isoforms and several enamel proteins. TGF-β isoforms significantly upregulated the mRNA level of gene expression of related genes for maturation-stage ameloblasts in mHAT9d cells. Furthermore, the total number of caspase-3-positive apoptotic events and amount of ssDNA were significantly rose and there was the homogeny about their distribution in three TGF-β isoforms. TGF-β is regulated in an isoform-specific manner to exert multiple biological functions such as cell proliferation, gene expression and apoptosis in maturation-stage ameloblasts.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10007_description_14":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"・2018(平成30)年度 科学研究費補助金 研究活動スタート支援 研究成果報告書","subitem_description_language":"ja","subitem_description_type":"Other"},{"subitem_description":"・研究期間 (年度)：2017-08-25 – 2019-03-31","subitem_description_language":"ja","subitem_description_type":"Other"},{"subitem_description":"・研究分野：小児歯科学","subitem_description_language":"ja","subitem_description_type":"Other"},{"subitem_description":"・研究成果の学術的意義や社会的意義 : 本研究ではエナメル質形成過程におけるＴＧＦ-βの役割について追究を行った。TGF-βがアポトーシス誘導能を有する可能性を示唆するだけでなく、アイソフォーム特異的な発現の時期や分布があること、さらにそれぞれに違う役割を有している可能性を見出した。生理活性物質に注目した研究は、正常なエナメル質の形成メカニズムを知ることのみならず、歯周組織やエナメル質の再生医療への大きな足掛かりになると期待している。","subitem_description_language":"ja","subitem_description_type":"Other"}]},"item_10007_description_9":{"attribute_name":"研究課題番号","attribute_value_mlt":[{"subitem_description":"研究課題/領域番号 : 17H07198","subitem_description_language":"ja","subitem_description_type":"Other"}]},"item_10007_relation_17":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-17H07198/","subitem_relation_type_select":"URI"}}]},"item_10007_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-03-16"}],"displaytype":"detail","filename":"17H07198.pdf","filesize":[{"value":"116.1 kB"}],"format":"application/pdf","licensetype":"license_6","mimetype":"application/pdf","url":{"label":"17H07198","url":"https://tsurumi-u.repo.nii.ac.jp/record/1051/files/17H07198.pdf"},"version_id":"6386979a-fe6e-4a6e-8edb-03d4fb234742"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"TGF-β","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"エナメル質","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"エナメルタンパク質","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"アポトーシス","subitem_subject_language":"ja","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_researcher":{"attribute_name":"研究代表者","attribute_type":"creator","attribute_value_mlt":[{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"","affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"http://www.isni.org/isni/"}],"affiliationNames":[{"affiliationName":"","affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"KOBAYASHI, Saeko","creatorNameLang":"en"},{"creatorName":"小林, 冴子","creatorNameLang":"ja"},{"creatorName":"コバヤシ, サエコ","creatorNameLang":"ja-Kana"}],"familyNames":[{"familyName":"KOBAYASHI","familyNameLang":"en"},{"familyName":"小林","familyNameLang":"ja"},{"familyName":"コバヤシ","familyNameLang":"ja-Kana"}],"givenNames":[{"givenName":"Saeko","givenNameLang":"en"},{"givenName":"冴子","givenNameLang":"ja"},{"givenName":"サエコ","givenNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"797","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"90804534","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=90804534"},{"nameIdentifier":"797","nameIdentifierScheme":"WEKO著者ID"}]}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"TGF-βによるエナメル芽細胞の分化誘導及びアポトーシス誘導研究","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"TGF-βによるエナメル芽細胞の分化誘導及びアポトーシス誘導研究","subitem_title_language":"ja"},{"subitem_title":"Induction of apoptosis by TGF-beta isoforms in amelogenesis","subitem_title_language":"en"}]},"item_type_id":"10007","owner":"10","path":["160"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2022-03-16"},"publish_date":"2022-03-16","publish_status":"0","recid":"1051","relation_version_is_last":true,"title":["TGF-βによるエナメル芽細胞の分化誘導及びアポトーシス誘導研究"],"weko_creator_id":"10","weko_shared_id":-1},"updated":"2024-08-01T23:20:46.505182+00:00"}