{"created":"2023-05-15T08:55:47.599526+00:00","id":1018,"links":{},"metadata":{"_buckets":{"deposit":"303c0461-46e1-4716-8113-89cf1c4c9a06"},"_deposit":{"created_by":10,"id":"1018","owners":[10],"pid":{"revision_id":0,"type":"depid","value":"1018"},"status":"published"},"_oai":{"id":"oai:tsurumi-u.repo.nii.ac.jp:00001018","sets":["100:101:103:157"]},"author_link":["775"],"item_10007_date_8":{"attribute_name":"報告年度","attribute_value_mlt":[{"subitem_date_issued_datetime":"2020-03-31","subitem_date_issued_type":"Issued"}]},"item_10007_description_13":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"研究成果の概要（和文）：体外から細胞内に吸収されたALAはPpIXに変換され、青紫色励起光で赤色蛍光を発する。癌細胞ではPpIX が細胞内に蓄積することを応用し、口腔表在癌を疑う患者の病変部にALA含浸ガーゼを貼付後、小型診断装置にて赤色蛍光を観測し色度分析した。病変中の赤色成分の平均値を代表値としてROC曲線で解析したところ、中等度上皮性異形成以上の病変を感度、特異度とも極めて良好に検出可能であった。一部の症例で、ALAの細胞内取込みに関与するPEPT1およびPpⅨの細胞外排出に関与するDynaminとABCG2について免疫組織学的染色を行ったが、これらの反応については健常部と異形成や癌部との差がみられなかった。","subitem_description_language":"ja","subitem_description_type":"Abstract"},{"subitem_description":"研究成果の概要（英文）：Absorbed extracellular 5-amynolevulinic acid（ALA）is metabolized to protoporphyrin Ⅸ（PpⅨ）which exhibits red fluorescence excited by bluish purple light. Utilizing the property that cancer cells intracellularly accumulate PpⅨ, ALA was topically applied to the patients who was clinically suspected superficial oral cancer, then observed red fluorescence using newly developed mobile detection device. X（red）element of chromaticity in the digital images obtained from the device were measured. ROC curve drawn by the mean value of X element in each lesion could discriminate moderate dysplasia and more from mild dysplasia or non-dysplasia with high sensitivity and high specificity. Some of the cases were examined immunohistochemically using antibodies against PEPT1 which transport ALA into the cytoplasm, Dynamin and ABCG2 which play roles of transportation of PpⅨ. However, the difference between dysplastic epithelium and surrounding normal epithelium was not found.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10007_description_14":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"・2019(令和元)年度 科学研究費補助金 基盤研究(C) 研究成果報告書","subitem_description_language":"ja","subitem_description_type":"Other"},{"subitem_description":"・研究期間 (年度)：2017-04-01 – 2020-03-31","subitem_description_language":"ja","subitem_description_type":"Other"},{"subitem_description":"・研究分野：口腔外科","subitem_description_language":"ja","subitem_description_type":"Other"},{"subitem_description":"・研究成果の学術的意義や社会的意義 : ALAを用いた光線力学的診断は脳腫瘍や膀胱癌などで既に応用されてはいるが、赤色蛍光を色度で数値化した本研究により、癌化傾向の高い中等度から高度の上皮性異形成も含めた口腔表在癌に対して極めて高い感度と特異度で診断できることが分かった。また、ALAは基本的に為害性がなく局所貼付でも内服や静脈内注射と同様に応用できることがわかったので、今回開発した手軽に持ち運べる小型診断装置と相まって、炎症性病変や反応性病変と区別しにくい口腔表在癌やその前駆病変のスクリーニング検査として優れた方法となりえる。また今後病変の蛍光画像を大量集積することで、その蛍光パターンからAI診断も可能になると考えられる。","subitem_description_language":"ja","subitem_description_type":"Other"}]},"item_10007_description_9":{"attribute_name":"研究課題番号","attribute_value_mlt":[{"subitem_description":"研究課題/領域番号 : 17K11889","subitem_description_language":"ja","subitem_description_type":"Other"}]},"item_10007_relation_17":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-17K11889/","subitem_relation_type_select":"URI"}}]},"item_10007_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-03-08"}],"displaytype":"detail","filename":"17K11889.pdf","filesize":[{"value":"658.9 kB"}],"format":"application/pdf","licensetype":"license_6","mimetype":"application/pdf","url":{"label":"17K11889","url":"https://tsurumi-u.repo.nii.ac.jp/record/1018/files/17K11889.pdf"},"version_id":"ec255565-9cef-478f-80fc-32eb79dfa258"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"口腔表在癌","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"光線力学的診断","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"5-アミノレブリン酸","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"プロトポルフィリンIX","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"色度","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"PEPT1","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"ABCG2","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Dynamin","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_researcher":{"attribute_name":"研究代表者","attribute_type":"creator","attribute_value_mlt":[{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"","affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"http://www.isni.org/isni/"}],"affiliationNames":[{"affiliationName":"","affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"SATO, Toru","creatorNameLang":"en"},{"creatorName":"佐藤, 徹","creatorNameLang":"ja"},{"creatorName":"サトウ, トオル","creatorNameLang":"ja-Kana"}],"familyNames":[{"familyName":"SATO","familyNameLang":"en"},{"familyName":"佐藤","familyNameLang":"ja"},{"familyName":"サトウ","familyNameLang":"ja-Kana"}],"givenNames":[{"givenName":"Toru","givenNameLang":"en"},{"givenName":"徹","givenNameLang":"ja"},{"givenName":"トオル","givenNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"775","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"30170765","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=30170765"},{"nameIdentifier":"775","nameIdentifierScheme":"WEKO著者ID"}]}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"5-ALAを用いた口腔癌の蛍光診断および術中蛍光ナビゲーションシステムの開発","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"5-ALAを用いた口腔癌の蛍光診断および術中蛍光ナビゲーションシステムの開発","subitem_title_language":"ja"},{"subitem_title":"Development of photodynamic diagnosis and intra-operative navigation system for oral cancer using 5-ALA","subitem_title_language":"en"}]},"item_type_id":"10007","owner":"10","path":["157"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2022-03-08"},"publish_date":"2022-03-08","publish_status":"0","recid":"1018","relation_version_is_last":true,"title":["5-ALAを用いた口腔癌の蛍光診断および術中蛍光ナビゲーションシステムの開発"],"weko_creator_id":"10","weko_shared_id":-1},"updated":"2024-08-01T08:46:55.491689+00:00"}