{"created":"2023-05-15T08:55:47.354846+00:00","id":1013,"links":{},"metadata":{"_buckets":{"deposit":"6f101497-d42f-490a-8945-b166349f9cbe"},"_deposit":{"created_by":10,"id":"1013","owners":[10],"pid":{"revision_id":0,"type":"depid","value":"1013"},"status":"published"},"_oai":{"id":"oai:tsurumi-u.repo.nii.ac.jp:00001013","sets":["100:101:103:157"]},"author_link":["770"],"item_10007_date_8":{"attribute_name":"報告年度","attribute_value_mlt":[{"subitem_date_issued_datetime":"2020-03-31","subitem_date_issued_type":"Issued"}]},"item_10007_description_13":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"研究成果の概要（和文）：歯髄には間葉系幹細胞が存在することが知られているが、特性は明らかになっていない。近年、報告されたMuse細胞はあらゆる間葉系組織に存在すると報告されているものの、口腔組織中に存在するとの報告は少ない。そこで本研究は、歯髄組織中にMuse細胞が存在しているか検討した。歯髄細胞より分離したSSEA-3陽性細胞は約0.5%であり、その細胞は3胚葉性の遺伝子発現に加え自己複製能を示した。また、歯髄細胞由来SSEA-3陽性細胞を石灰化誘導培地で分化させたところ、高い石灰化誘導能を示した。この結果から、ヒト歯髄組織由来SSEA-3陽性細胞は骨形成能を有する細胞に分化する能力が高いことが示唆された。","subitem_description_language":"ja","subitem_description_type":"Abstract"},{"subitem_description":"研究成果の概要（英文）：Mesenchymal stem cells are known to exist in pulp tissue. However, the detailed characteristics of the stem cells have not been elucidated. Recently, the presence of novel Muse cells which has been reported. Researches mentioned that Muse cells exist in all mesenchymal tissues. However, there is hardly any report regarding the existence of Muse cells in oral tissues. Therefore, the purpose of this study was to determine the presence of Muse cells in dental pulp tissue. About 0.5% of SSEA-3 positive cells were isolated from dental pulp cells by cell sorter. The isolated cells showed self-renewal ability in addition to expressing the genes representing the three germ layers. Furthermore, the isolated cells showed a high calcification ability when subjected to calcification-inducing medium. The results suggest that SSEA-3 positive cells derived from human dental pulp tissue are similar to Muse cells, highly capable of differentiating into osteogenic cells.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10007_description_14":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"・2019(令和元)年度 科学研究費補助金 基盤研究(C) 研究成果報告書","subitem_description_language":"ja","subitem_description_type":"Other"},{"subitem_description":"・研究期間 (年度)：2016-10-21 – 2020-03-31","subitem_description_language":"ja","subitem_description_type":"Other"},{"subitem_description":"・研究分野：歯周病学","subitem_description_language":"ja","subitem_description_type":"Other"},{"subitem_description":"・研究成果の学術的意義や社会的意義 : Muse細胞は、表面抗原であるCD105とSSEA-3の抗体を用いて二重染色後、ソーティングを行い得られるが、SSEA-3単独での分離を行った細胞でも一定程度の幹細胞が得られ、その細胞は石灰化能を有することが本研究により明らかとなった。歯髄組織は比較的低侵襲で細胞を得ることがで可能で、便宜抜歯や智歯抜歯で得られる抜去歯は医療廃棄物であり、それらの抜去歯から歯髄組織を得ることができる。現在、主に骨髄細胞を用いた再生医療が歯科領域では行われているが、それと比較してもより低侵襲で細胞を得られるメリットがあり、今後も新たな細胞供給源として歯髄組織の有効性を検証することが期待される。","subitem_description_language":"ja","subitem_description_type":"Other"}]},"item_10007_description_9":{"attribute_name":"研究課題番号","attribute_value_mlt":[{"subitem_description":"研究課題/領域番号 : 16K11844","subitem_description_language":"ja","subitem_description_type":"Other"}]},"item_10007_relation_17":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-16K11844/","subitem_relation_type_select":"URI"}}]},"item_10007_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-03-08"}],"displaytype":"detail","filename":"16K11844.pdf","filesize":[{"value":"278.7 kB"}],"format":"application/pdf","licensetype":"license_6","mimetype":"application/pdf","url":{"label":"16K11844","url":"https://tsurumi-u.repo.nii.ac.jp/record/1013/files/16K11844.pdf"},"version_id":"8b619641-856e-4ae8-8c52-8536118277dc"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"歯髄","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"幹細胞","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"SSEA-3","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"フローサイトメトリー","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"歯周組織再生療法","subitem_subject_language":"ja","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_researcher":{"attribute_name":"研究代表者","attribute_type":"creator","attribute_value_mlt":[{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"","affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"http://www.isni.org/isni/"}],"affiliationNames":[{"affiliationName":"","affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"NAGANO, Takatoshi","creatorNameLang":"en"},{"creatorName":"長野, 孝俊","creatorNameLang":"ja"},{"creatorName":"ナガノ, タカトシ","creatorNameLang":"ja-Kana"}],"familyNames":[{"familyName":"NAGANO","familyNameLang":"en"},{"familyName":"長野","familyNameLang":"ja"},{"familyName":"ナガノ","familyNameLang":"ja-Kana"}],"givenNames":[{"givenName":"Takatoshi","givenNameLang":"en"},{"givenName":"孝俊","givenNameLang":"ja"},{"givenName":"タカトシ","givenNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"770","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"10386914","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=10386914"},{"nameIdentifier":"770","nameIdentifierScheme":"WEKO著者ID"}]}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"歯髄由来間葉系細胞中Muse細胞の特性解析と歯周組織再生医療応用への展開","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"歯髄由来間葉系細胞中Muse細胞の特性解析と歯周組織再生医療応用への展開","subitem_title_language":"ja"},{"subitem_title":"Analysis of the Muse cells characteristics of were obtained from dental pulp, and development of peridontal regerative therapy","subitem_title_language":"en"}]},"item_type_id":"10007","owner":"10","path":["157"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2022-03-08"},"publish_date":"2022-03-08","publish_status":"0","recid":"1013","relation_version_is_last":true,"title":["歯髄由来間葉系細胞中Muse細胞の特性解析と歯周組織再生医療応用への展開"],"weko_creator_id":"10","weko_shared_id":-1},"updated":"2024-08-01T08:37:22.052217+00:00"}