{"created":"2023-05-15T08:55:46.876915+00:00","id":1003,"links":{},"metadata":{"_buckets":{"deposit":"0b223c0d-562b-47ea-ba17-5a65af73aa4a"},"_deposit":{"created_by":10,"id":"1003","owners":[10],"pid":{"revision_id":0,"type":"depid","value":"1003"},"status":"published"},"_oai":{"id":"oai:tsurumi-u.repo.nii.ac.jp:00001003","sets":["100:101:103:149"]},"author_link":["761"],"control_number":"1003","item_10007_date_8":{"attribute_name":"報告年度","attribute_value_mlt":[{"subitem_date_issued_datetime":"2021-03-31","subitem_date_issued_type":"Issued"}]},"item_10007_description_13":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"研究成果の概要（和文）： 本研究はヒストンメチル化酵素G9aが細胞増殖関連遺伝子の統合的な発現制御機構を明らかにする事を目的とした。その結果、G9a欠損細胞ではRunx2発現が変動せず、Runx2制御化のFgfrs（増殖関連遺伝子）、Osteocalcin（分化マーカー遺伝子）の発現が抑制される事、Fgfrs、Osteocalcinの転写調節領域にG9aとRunx2が共に結合する事、内在性のG9aとRunx2が結合する事、G9aがRunx2の転写活性化能を亢進する事、が明らかになった。以上から、G9aが前駆骨芽細胞の増殖・分化に関わる遺伝子の転写調節をつかさどるRunx2の転写制御複合体の一員である可能性が示唆された。","subitem_description_language":"ja","subitem_description_type":"Abstract"},{"subitem_description":"研究成果の概要（英文）：In this study, we aimed to elucidate how histone methyltransferase G9a integrally regulates the expression of proliferation-related genes in osteoblast progenitors at the osteogenic front. We found that the expression of Runx2 was not altered, but the expression of Runx2-regulated Fgfrs (proliferation-related genes) and Osteocalcin (differentiation marker genes) was repressed in G9a-deficient cells. Both G9a and Runx2 bound to the transcriptional regulatory regions of Fgfrs and Osteocalcin, and endogenous G9a bound to Runx2, indicating that G9a enhanced the transcriptional activation of Runx2. These results suggest that G9a may be a member of the transcriptional regulatory complex of Runx2, which regulates transcription of genes involved in proliferation and differentiation of progenitor osteoblasts.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10007_description_14":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"・2020(令和2)年度 科学研究費補助金 若手研究(B) 研究成果報告書","subitem_description_language":"ja","subitem_description_type":"Other"},{"subitem_description":"・研究期間 (年度)：2017-04-01 – 2021-03-31","subitem_description_language":"ja","subitem_description_type":"Other"},{"subitem_description":"・研究分野：分子生物学","subitem_description_language":"ja","subitem_description_type":"Other"},{"subitem_description":"・研究成果の学術的意義や社会的意義 : ヒストンメチル化酵素G9aは元来、ヒストン H3、9番目リジン残基(H3K9)のモノメチル化(me1)とジメチル化(me2)を担うヒストン修飾酵素として同定された。さらにヒストン以外の機能性タンパク質のメチル化を担う事も報告されてきた。本研究では、G9aがRunx2による転写複合体に含まれており機能的な役割を担う、ヒストン修飾酵素以外の新たな点が示唆された。","subitem_description_language":"ja","subitem_description_type":"Other"}]},"item_10007_description_9":{"attribute_name":"研究課題番号","attribute_value_mlt":[{"subitem_description":"研究課題/領域番号 : 17K18196","subitem_description_language":"ja","subitem_description_type":"Other"}]},"item_10007_relation_17":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-17K18196/","subitem_relation_type_select":"URI"}}]},"item_10007_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-03-02"}],"displaytype":"detail","filename":"17K18196.pdf","filesize":[{"value":"83.0 kB"}],"format":"application/pdf","licensetype":"license_6","mimetype":"application/pdf","url":{"label":"17K18196","url":"https://tsurumi-u.repo.nii.ac.jp/record/1003/files/17K18196.pdf"},"version_id":"8b4d9963-90f7-4763-8ea4-28fd6985a327"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"G9a","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Runx2","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"骨芽細胞分化","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"osteogenic front","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_researcher":{"attribute_name":"研究代表者","attribute_type":"creator","attribute_value_mlt":[{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"","affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"http://www.isni.org/isni/"}],"affiliationNames":[{"affiliationName":"","affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"IDENO, Hisashi","creatorNameLang":"en"},{"creatorName":"出野, 尚","creatorNameLang":"ja"},{"creatorName":"イデノ, ヒサシ","creatorNameLang":"ja-Kana"}],"familyNames":[{"familyName":"IDENO","familyNameLang":"en"},{"familyName":"出野","familyNameLang":"ja"},{"familyName":"イデノ","familyNameLang":"ja-Kana"}],"givenNames":[{"givenName":"Hisashi","givenNameLang":"en"},{"givenName":"尚","givenNameLang":"ja"},{"givenName":"ヒサシ","givenNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"761","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"40435699","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=40435699"},{"nameIdentifier":"761","nameIdentifierScheme":"WEKO著者ID"}]}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"G9aによるosteogenic frontの増殖と分化の制御","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"G9aによるosteogenic frontの増殖と分化の制御","subitem_title_language":"ja"},{"subitem_title":"Regulation of differentiation and proliferation at osteogenic front by G9a","subitem_title_language":"en"}]},"item_type_id":"10007","owner":"10","path":["149"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2022-03-02"},"publish_date":"2022-03-02","publish_status":"0","recid":"1003","relation_version_is_last":true,"title":["G9aによるosteogenic frontの増殖と分化の制御"],"weko_creator_id":"10","weko_shared_id":-1},"updated":"2024-08-01T08:15:24.975769+00:00"}