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小児の精神的長期ストレスをモニターする唾液microRNAの同定
https://tsurumi-u.repo.nii.ac.jp/records/1260
https://tsurumi-u.repo.nii.ac.jp/records/126086f1b556-7710-4dc7-a446-5e4010c785b6
名前 / ファイル | ライセンス | アクション |
---|---|---|
24659919 (462.6 kB)
|
Item type | 報告書 / Research Paper(1) | |||||
---|---|---|---|---|---|---|
公開日 | 2022-05-09 | |||||
タイトル | ||||||
言語 | ja | |||||
タイトル | 小児の精神的長期ストレスをモニターする唾液microRNAの同定 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Identification of salivary microRNA which monitors pediatric prolonged mental stresses | |||||
言語 | ||||||
言語 | jpn | |||||
キーワード | ||||||
言語 | ja | |||||
主題Scheme | Other | |||||
主題 | ストレス | |||||
キーワード | ||||||
言語 | ja | |||||
主題Scheme | Other | |||||
主題 | 小児 | |||||
キーワード | ||||||
言語 | ja | |||||
主題Scheme | Other | |||||
主題 | う蝕 | |||||
キーワード | ||||||
言語 | ja | |||||
主題Scheme | Other | |||||
主題 | 唾液 | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_18ws | |||||
資源タイプ | research report | |||||
研究代表者 |
前田, 伸子
× 前田, 伸子 |
|||||
報告年度 | ||||||
日付 | 2014-03-31 | |||||
日付タイプ | Issued | |||||
研究課題番号 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 研究課題/領域番号 : 24659919 | |||||
言語 | ja | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | 研究成果の概要(和文):小児の精神的ストレスを非侵襲的に診断する方法を確立することを目的とし、バイオマーカーとなる唾液microRNA検索を試みた。被験者として、小学校児童1-5年生の問題を抱えカウンセリングした児童30名をストレス群、安定している児童30名をコントロール群とした。う蝕経験歯数はストレス群がコントロール群の2倍高かったが、う蝕関連菌数と唾液分泌量・緩衝能には差がなかった。そこで唾液microRNA抽出・精製方法を確立し、アレイで発現プロファイルを比較したところ、10種のmicroRNAにおいて発現パターンが異なり、クラスターマーカーとして有用である可能性が示唆された。 | |||||
言語 | ja | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | 研究成果の概要(英文):For the purpose to establish a diagnostic method for a child's psychological stress noninvasively, a survey of the salivary microRNA as a biomarker was carried out. The subjects were the elementary school juvenile first-fifth graders. In the subjects, 30 children who had and counseled problem were classified into the stress group, and 30 stable children were into the control group. Although the dental caries experience number of teeth was twice higher in the stress group than the control group, there was no difference in the number of cariogenic pathogens, the amount of salivary secretion and saliva buffer capacity. Then, the saliva microRNA extraction / purification methods were established and the expression profile was compared using an array. The expression patterns differed in ten sorts of microRNAs. The result suggested that microRNAs are useful as a cluster marker for diagnosis of children's psychological stress. | |||||
言語 | en | |||||
内容記述 | ||||||
内容記述タイプ | Other | |||||
内容記述 | ・2013(平成25)年度 科学研究費補助金 挑戦的萌芽研究 研究成果報告書 | |||||
言語 | ja | |||||
内容記述 | ||||||
内容記述タイプ | Other | |||||
内容記述 | ・研究期間 (年度):2012-04-01 – 2014-03-31 | |||||
言語 | ja | |||||
内容記述 | ||||||
内容記述タイプ | Other | |||||
内容記述 | ・研究分野:医歯薬学 | |||||
言語 | ja | |||||
関連サイト | ||||||
識別子タイプ | URI | |||||
関連識別子 | https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-24659919/ | |||||
著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa |